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Thermo Fisher
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Interleukin-15-Cultured Dendritic Cells Enhance Anti-Tumor Gamma Delta T Cell Functions through IL-15 Secretion
doi: 10.3389/fimmu.2018.00658
Figure Lengend Snippet: γδ T cell phenotype of healthy donors after 48 hours of culture with IPP, IL-15 dendritic cells (DCs) or both.
Article Snippet: γδ T cells were phenotyped after 48-hour co-culture using the following monoclonal antibodies (mAbs): γδ TCR-FITC (Miltenyi), CD86-FITC,
Techniques:
Journal: Frontiers in Immunology
Article Title: Interleukin-15-Cultured Dendritic Cells Enhance Anti-Tumor Gamma Delta T Cell Functions through IL-15 Secretion
doi: 10.3389/fimmu.2018.00658
Figure Lengend Snippet: γδ T cell phenotype of acute myeloid leukemia patients 48-hours after co-culture with IPP, IL-15 dendritic cells (DCs) or both.
Article Snippet: γδ T cells were phenotyped after 48-hour co-culture using the following monoclonal antibodies (mAbs): γδ TCR-FITC (Miltenyi), CD86-FITC,
Techniques:
Journal: BMC Medicine
Article Title: Targeting insulin resistance in type 2 diabetes via immune modulation of cord blood-derived multipotent stem cells (CB-SCs) in stem cell educator therapy: phase I/II clinical trial
doi: 10.1186/1741-7015-11-160
Figure Lengend Snippet: Anti-inflammatory effects of stem cell educator therapy. (A) Up-regulation of plasma levels of TGF-β1 in T2D patients at baseline and four weeks after Stem Cell Educator therapy. (B) Flow analysis of intra-cellular cytokines demonstrating differential effects on key interleukins at four weeks post-treatment. (C) Down-regulation percentage of CD86 + CD14 + monocytes in T2D patients at baseline and four weeks after Stem Cell Educator therapy. (D) Flow Analysis of CD4 + CD25 + Foxp3 + Tregs demonstrating no change in the percentage of Tregs at four weeks post-treatment.
Article Snippet: For cell surface staining, cells were incubated with mouse anti-human monoclonal antibodies (
Techniques:
Journal: The Journal of allergy and clinical immunology
Article Title: LRP-1 Attenuates House Dust Mite-induced Eosinophilic Airway Inflammation by Suppressing Dendritic Cell-mediated Adaptive Immune Responses
doi: 10.1016/j.jaci.2017.10.044
Figure Lengend Snippet: A) The percentage of CD11c+/Siglec F−/MHC IIhi total lung DCs and B) the percentage of lung DC monocyte-derived dendritic cells (moDCs), conventional myeloid dendritic cells (cDCs), CD103+ DCs, and plasmacytoid dendritic cells (pDCs) subsets as quantified by flow cytometry (P = NS, HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice, n= 9 mice per group, unpaired t test). C) Cell surface expression of CD80 and CD86 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 8 – 9 mice per group, * P< 0.01, Mann-Whitney test). D) Cell surface expression of Dectin1, Dectin 2, CD205, CD206, and DC-SIGN by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n = 4 – 9 mice per group, * P = 0.029, Mann-Whitney test). E) Cell surface expression of TLR4 and TLR5 by lung CD11c+/CD11b+/SSClo/SiglecF−/MHC IIhi DCs from HDM-challenged Lrp1fl/fl; CD11c-Cre versus Lrp1fl/fl mice (n= 4 – 9 mice per group, *P < 0.05, unpaired t test).
Article Snippet: Mouse DC subsets were identified and characterized by using anti- mouse Siglec F-PE-CF594, CD11c-APC Cy7, MHCII-PE Cy7, CD11b-PerCP Cy5.5, CD103- APC, PDCA1-AF488, Mar-1-efluor450, CD64-PE, TLR-4-APC, Dectin-1-PE, CD205- PerCPCy5.5, and CD206-BV785 antibodies, all from BioLegend (San Diego, CA);
Techniques: Derivative Assay, Flow Cytometry, Expressing, MANN-WHITNEY